That does it! I have had enough! [slowly walks up to the camera] They can take the suit off your back. They can take your special cereal bowl and spoon. They can even take your album of photo memories. [at this point, his face is very close to the camera] But when they mess with your dots... they go too far.
S5 E2Mystery
S5 E10Playhouse for Sale
[Last lines]
[after Pee-Wee has gotten on his scooter] Pee-Wee, where are you going?
[to Miss Yvonne] To get some lemons! [he chuckles, and looks at the screen for the final time] The playhouse will always be here for everyone to play in, forever, and ever, and ever. And on that, you have my word!
[Everyone screams the very last time. Pee-wee scoots to the supermarket, ending the episode and the series]
These quotes come from Wikiquote's Pee-wee's Playhouse pages and are available under the Creative Commons Attribution-ShareAlike License.

